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El-Zohri, M. H. A. ; Čabala, Radomír ; Frank, Hartmut:
Quantification of phytochelatins in plants by reversed-phase HPLC-ESI-MS-MS.
In: Analytical and Bioanalytical Chemistry.
Bd. 382
(2005)
Heft 8
.
- S. 1871-1876.
ISSN 1618-2650
DOI: https://doi.org/10.1007/s00216-005-3331-0
Abstract
An on-line HPLC-ESI-MS-MS method has been developed for determination of glutathione and phytochelatins (PC) in plant tissues. For sample pre-treatment, dithiothreitol (DTT) must be added at the very beginning, as an anti-oxidant. Optimization of instrumental conditions i.e. composition of HPLC mobile phase, ionization efficiency of the electrospray interface, and MS-MS detection in the multiple ion-monitoring mode, are the central aspects of this work. A polystyrene-packed column was found to be superior to a standard silica-packed reversed-phase column. A concave quadratic gradient of ammonium formate buffer and acetonitrile was found to be optimum. The limits of quantitation were 0.2 mu mol kg(-1) plant tissue for glutathione and PC. The method has been applied to analysis of tissue samples from Vicia faba grown in Cd-containing nutrient solutions.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Zusätzliche Informationen: | BAYCEER33883 |
| Institutionen der Universität: | Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER Forschungseinrichtungen Forschungseinrichtungen > Forschungszentren |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik |
| Eingestellt am: | 13 Aug 2015 06:12 |
| Letzte Änderung: | 13 Aug 2015 06:12 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/17988 |