at Google ScholarTitle data
Humenik, Martin ; Smith, Andrew M. ; Arndt, Sina ; Scheibel, Thomas:
Ion and seed dependent fibril assembly of a spidroin core domain.
In: Journal of Structural Biology.
Vol. 191
(2015)
Issue 2
.
- pp. 130-138.
ISSN 1047-8477
DOI: https://doi.org/10.1016/j.jsb.2015.06.021
Abstract in another language
Recombinant eADF4(C16) represents an engineered spider silk variant based on the sequence of the core
domain of the natural dragline silk protein ADF4 of Araneus diadematus. Previously eADF4(C16) has been
shown to self-assemble into cross-b fibrils in a two-step process of nucleus formation and fibril growth.
Here, it is shown that structurally converted low molecular weight oligomers can act as nuclei. Further, it
could be determined that specifically potassium and phosphate ions strongly influence both nucleus
formation as well as fibril growth. Nucleation of fibril assembly could be surpassed by seeding soluble
protein with pre-assembled fibrils but also, unexpectedly, with eADF4(C16) sub-micrometer particles.
The latter finding reveals that spider silk fibril assembly seems to be rather dependent on the protein
sequence than on the structural features, since cross-seeding with other proteins was not possible.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | Fibril Nucleus Particle Recombinant spider silk seed |
| Institutions of the University: | Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Profile Fields > Advanced Fields > Advanced Materials Profile Fields > Advanced Fields > Molecular Biosciences Profile Fields > Advanced Fields > Polymer and Colloid Science Profile Fields > Emerging Fields > Food and Health Sciences Profile Fields Profile Fields > Advanced Fields Profile Fields > Emerging Fields |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 21 Sep 2015 12:39 |
| Last Modified: | 15 Jul 2022 07:27 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/19465 |