at Google ScholarTitle data
Vendrely, Charlotte ; Ackerschott, Christian B. ; Römer, Lin ; Scheibel, Thomas:
Molecular design of performance proteins with repetitive sequences: recombinant flagelliform spider silk as basis for biomaterials.
In: Gazit, Ehud ; Nussinov, Ruth
(ed.):
Nanostructure design : methods and protocols. -
Totowa, NJ
: Humana Press
,
2008
. - pp. 3-14
. - (Methods in Molecular Biology
; 474
)
ISBN 978-1-934115-35-0
DOI: https://doi.org/10.1007/978-1-59745-480-3_1
Abstract in another language
Most performance proteins responsible for the mechanical stability of cells and organisms reveal highly repetitive sequences. Mimicking such performance proteins is of high interest for the design of nanostructured biomaterials. In this article, flagelliform silk is exemplary introduced to describe a general principle for designing genes of repetitive performance proteins for recombinant expression in Escherichia coli . In the first step, repeating amino acid sequence motifs are reversely transcripted into DNA cassettes, which can in a second step be seamlessly ligated, yielding a designed gene. Recombinant expression thereof leads to proteins mimicking the natural ones. The recombinant proteins can be assembled into nanostructured materials in a controlled manner, allowing their use in several applications.
Further data
| Item Type: | Article in a book |
|---|---|
| Refereed: | Yes |
| Keywords: | Biomaterials; recombinant production; repetitive sequence; spider silk proteins |
| Institutions of the University: | Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials Profile Fields > Advanced Fields > Advanced Materials Profile Fields > Advanced Fields > Molecular Biosciences Profile Fields > Advanced Fields > Polymer and Colloid Science Profile Fields > Emerging Fields > Food and Health Sciences Profile Fields Profile Fields > Advanced Fields Profile Fields > Emerging Fields |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 23 Sep 2015 12:28 |
| Last Modified: | 23 Apr 2024 10:55 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/19496 |