Title data
Geisler, Michael ; Pirzer, Tobias ; Ackerschott, Christian B. ; Lud, Simon Q. ; Garrido, Jose A. ; Scheibel, Thomas ; Hugel, Thorsten:
Hydrophobic and Hofmeister Effects on the Adhesion of Spider Silk Proteins onto Solid Substrates: An AFM-Based Single-Molecule Study.
In: Langmuir.
Vol. 24
(2008)
Issue 4
.
- pp. 1350-1355.
ISSN 1520-5827
DOI: https://doi.org/10.1021/la702341j
Abstract in another language
AFM-based single-molecule force spectroscopy has been used to study the effect of Hofmeister salts and protein hydrophobicity on the adhesion of recombinant spider silk proteins onto solid substrates. Therefore, a molecular probe consisting of a spider silk protein and an AFM tip has been developed, which (i) is a well-defined, small system that can be simulated by molecular dynamics simulations, (ii) allows access to the whole soluble concentration range for ions, and (iii) provides the distribution of desorption forces rather than just ensemble-averaged mean values. The measured desorption forces follow the Hofmeister series for anions (H2PO4-, Cl-, I-) with a stabilizing energy of more than 15 kBT for 5 M NaH2PO4. Moreover, this effect is influenced by the hydrophobicity of the spider silk protein, indicating that hydrophobic and Hofmeister effects are closely related.
Further data
Item Type: | Article in a journal |
---|---|
Refereed: | Yes |
Institutions of the University: | Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Profile Fields > Advanced Fields > Advanced Materials Profile Fields > Advanced Fields > Molecular Biosciences Profile Fields > Advanced Fields > Polymer and Colloid Science Profile Fields > Emerging Fields > Food and Health Sciences Profile Fields Profile Fields > Advanced Fields Profile Fields > Emerging Fields |
Result of work at the UBT: | Yes |
DDC Subjects: | 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering |
Date Deposited: | 24 Sep 2015 07:02 |
Last Modified: | 27 Jul 2022 13:30 |
URI: | https://eref.uni-bayreuth.de/id/eprint/19515 |