at Google ScholarTitle data
Schmidt, Martin ; Römer, Lin ; Strehle, Martin ; Scheibel, Thomas:
Conquering isoleucine auxotrophy of Escherichia coli BLR(DE3) to recombinantly produce spider silk proteins in minimal media.
In: Biotechnology Letters.
Vol. 29
(2007)
Issue 11
.
- pp. 1741-1744.
ISSN 1573-6776
DOI: https://doi.org/10.1007/s10529-007-9461-z
Abstract in another language
Large-scale production of recombinant spider silk proteins is a long-term goal for their industrial use. Therefore, we have recently developed a process for bacterial production. Due to a highly repetitive gene sequence of spider silks, the host strain E. coli BLR(DE3) was employed since it shows
no homologue recombination. Although perfectly suited for production in full media, the BLR strain does not grow in cost-effective minimal media, indicating a previously not reported L-isoleucine auxotrophy. We provide evidence that mutated threonine deaminase is likely responsible for the
detected auxotrophy of BLR.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | Cultivation; Repetitive sequence; Threonine deaminase |
| Institutions of the University: | Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Profile Fields > Advanced Fields > Advanced Materials Profile Fields > Advanced Fields > Molecular Biosciences Profile Fields > Advanced Fields > Polymer and Colloid Science Profile Fields > Emerging Fields > Food and Health Sciences Profile Fields Profile Fields > Advanced Fields Profile Fields > Emerging Fields |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 24 Sep 2015 08:39 |
| Last Modified: | 26 Nov 2015 10:51 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/19533 |