at Google ScholarTitle data
Daniel, Steven L. ; Pilsl, Christine ; Drake, Harold L.:
Oxalate metabolism by the acetogenic bacterium Moorella thermoacetica.
In: FEMS Microbiology Letters.
Vol. 231
(2004)
Issue 1
.
- pp. 39-43.
ISSN 1574-6968
DOI: https://doi.org/10.1016/S0378-1097(03)00924-8
Abstract in another language
Whole-cell and cell-extract experiments were performed to study the mechanism of oxalate metabolism in the acetogenic bacterium Moorella thermoacetica. In short-term, whole-cell assays, oxalate consumption was low unless cell suspensions were supplemented with CO2, KNO3, or Na2S2O3. Cell extracts catalyzed the oxalate-dependent reduction of benzyl viologen. Oxalate consumption occurred concomitant to benzyl viologen reduction; when benzyl viologen was omitted, oxalate was not appreciably consumed. Based on benzyl viologen reduction, specific activities of extracts averaged 0.6 µmol oxalate oxidized min-1 mg protein-1. Extracts also catalyzed the formate-dependent reduction of NADP+; however, oxalate-dependent reduction of NADP+ was negligible. Oxalate- or formate-dependent reduction of NAD+ was not observed. Addition of coenzyme A (CoA), acetyl-CoA, or succinyl-CoA to the assay had minimal effect on the oxalate-dependent reduction of benzyl viologen. These results suggest that oxalate metabolism by M. thermoacetica requires a utilizable electron acceptor and that CoA-level intermediates are not involved.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Additional notes: | BAYCEER21223 |
| Institutions of the University: | Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Research Institutions Research Institutions > Research Centres |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science |
| Date Deposited: | 24 Sep 2015 09:37 |
| Last Modified: | 24 Sep 2015 09:37 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/19554 |