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Use of the avidin (imino)biotin system as a general approach to affinity precipitation

Titelangaben

Freitag, Ruth ; Hilbrig, Frank:
Use of the avidin (imino)biotin system as a general approach to affinity precipitation.
In: McMahon, Robert J. (Hrsg.): Avidin-Biotin interactions : methods and applications. - Totowa, NJ : Humana Press , 2008 . - S. 35-50 . - (Methods in Molecular Biology ; 418 )
ISBN 978-1-588-29583-5

Abstract

Biospecific interactions are used in many capturing and bioseparation steps. A typical situation is the coupling of a biospecific ligand to a chromatographic stationary phase for affinity chromatography. This approach has two possible drawbacks. The first is that a chromatographic column may be awkward to use in experimental setups; the second is related to the need to develop a dedicated coupling chemistry for any given affinity ligand. In affinity precipitation, the biospecific affinity ligand is instead linked to a stimuli-responsive molecule to yield a so-called affinity macroligand (AML). Upon stimulation, such molecules show abrupt yet reversible precipitation from aqueous solution. Capture by affinity precipitation just requires the addition of the stimuli-responsive AML to the raw target solution followed by selective precipitation of the formed affinity complex via the application of the stimulus. The need for the synthesis of a dedicated AML may be circumvented by the use of an avidin-activated stimuli-responsive precursor, to which any biotinylated affinity ligand can be securely linked via the well-known strong avidin-biotin interaction.

Weitere Angaben

Publikationsform: Aufsatz in einem Buch
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik > Lehrstuhl Bioprozesstechnik - Univ.-Prof. Dr. Ruth Freitag
Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 24 Feb 2016 14:41
Letzte Änderung: 17 Mai 2016 07:12
URI: https://eref.uni-bayreuth.de/id/eprint/31040