at Google ScholarTitle data
Freitag, Ruth ; Hilbrig, Frank:
Use of the avidin (imino)biotin system as a general approach to affinity precipitation.
In: McMahon, Robert J.
(ed.):
Avidin-Biotin interactions : methods and applications. -
Totowa, NJ
: Humana Press
,
2008
. - pp. 35-50
. - (Methods in Molecular Biology
; 418
)
ISBN 978-1-588-29583-5
Abstract in another language
Biospecific interactions are used in many capturing and bioseparation steps. A typical situation is the coupling of a biospecific ligand to a chromatographic stationary phase for affinity chromatography. This approach has two possible drawbacks. The first is that a chromatographic column may be awkward to use in experimental setups; the second is related to the need to develop a dedicated coupling chemistry for any given affinity ligand. In affinity precipitation, the biospecific affinity ligand is instead linked to a stimuli-responsive molecule to yield a so-called affinity macroligand (AML). Upon stimulation, such molecules show abrupt yet reversible precipitation from aqueous solution. Capture by affinity precipitation just requires the addition of the stimuli-responsive AML to the raw target solution followed by selective precipitation of the formed affinity complex via the application of the stimulus. The need for the synthesis of a dedicated AML may be circumvented by the use of an avidin-activated stimuli-responsive precursor, to which any biotinylated affinity ligand can be securely linked via the well-known strong avidin-biotin interaction.
Further data
| Item Type: | Article in a book |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 24 Feb 2016 14:41 |
| Last Modified: | 17 May 2016 07:12 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/31040 |