Development of a fed-batch process for the production of a dye-linked formaldehyde dehydrogenase in Hyphomicrobium zavarzinii ZV 580

Title data

Jérôme, Valérie ; Hermann, Markus ; Hilbrig, Frank ; Freitag, Ruth:
Development of a fed-batch process for the production of a dye-linked formaldehyde dehydrogenase in Hyphomicrobium zavarzinii ZV 580.
In: Applied Microbiology and Biotechnology. Vol. 77 (2007) Issue 4 . - pp. 779-788.
ISSN 1432-0614
DOI: https://doi.org/10.1007/s00253-007-1218-z

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Abstract in another language

The dye-linked formaldehyde dehydrogenase (dlFalDH) from Hyphomicrobium zavarzinii ZV 580 processes formaldehyde in a highly selective manner and without need for NAD(P). The enzyme thus has considerable potential for technical applications if the difficulties associated with its efficient production can be resolved. In this contribution, a fed-batch bioprocess is developed, which improves both the biomass production of H. zavarzinii ZV 580 (from 0.6 to 2 g l(-1) dry mass) and the specific dlFalDH production (from 0.1 to 0.3 units g(-1) biomass), resulting in an overall improvement of the productivity by more than an order of magnitude compared to the previously reported process (Klein et al., Biochem J 301:289-295, 1994). In particular, the process uses an automated feeding strategy controlled via the dissolved oxygen concentration. In addition, our results show that the growth of H. zavarzinii ZV 580 is rather sensitive toward increasing salt concentration in the culture medium. Growth is also inhibited by the presence of surfactant-based antifoam reagents. Adjustment of the pH via the addition of methylamine instead of NaOH, on the other hand, leads to an increase in biomass yield.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties
Result of work at the UBT:	Yes
DDC Subjects:	600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited:	24 Feb 2016 15:11
Last Modified:	27 Oct 2022 07:08
URI:	https://eref.uni-bayreuth.de/id/eprint/31042