at Google ScholarTitle data
Yamahata, Christophe ; Vandevyver, Caroline ; Lacharme, Frederic ; Izewska, Paulina ; Vogel, Horst ; Freitag, Ruth ; Gijs, Martin A. M.:
Pumping of mammalian cells with a nozzle-diffuser micropump.
In: Lab on a Chip.
Vol. 5
(2005)
Issue 10
.
- pp. 1083-1088.
ISSN 1473-0189
DOI: https://doi.org/10.1039/B504468E
Abstract in another language
We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science 500 Science > 500 Natural sciences 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 25 Feb 2016 14:08 |
| Last Modified: | 25 Feb 2016 14:08 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/31052 |