Investigation of the steric mass action formalism in the simulation of breakthrough curves on a monolithic and a packed bed column

Title data

Jozwik, Magdalena ; Kaczmarski, Krzystof ; Freitag, Ruth:
Investigation of the steric mass action formalism in the simulation of breakthrough curves on a monolithic and a packed bed column.
In: Journal of Chromatography A. Vol. 1073 (2005) Issue 1-2 . - pp. 111-121.
ISSN 0021-9673
DOI: https://doi.org/10.1016/j.chroma.2005.01.059

Official URL:

Abstract in another language

The simulation of the behaviour of two proteins (cytochrom c and α-chymotrypsinogen) on two types of stationary phases (monolithic and porous particle-based) was attempted for non-linear (simulation of breakthrough curves) and linear (simulation of elution peaks) cation exchange chromatography. It was found that the combination of a stoichiometric model (steric mass action, SMA) with the lumped pore diffusion (POR) model allows a simulation of high predictive value. Using one set of SMA and transport parameters for a given column morphology and/or protein, breakthrough curves and peaks could be simulated that agreed well with the experimental data while no dependency on either the protein load or the mobile phase composition (salt content) was observed. One of the SMA parameters, namely the characteristic charge needed some slight adjustment (within the error of the experimental determination of this parameter) in order to optimise the fit.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Additional notes:	28th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2004)
Keywords:	POR
Institutions of the University:	Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT:	No
DDC Subjects:	600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited:	25 Feb 2016 14:28
Last Modified:	30 Jun 2022 10:15
URI:	https://eref.uni-bayreuth.de/id/eprint/31056