Title data
Freitag, Ruth:
Comparison of the chromatographic behavior of monolithic capillary columns in capillary electrochromatography and nano-high-performance liquid chromatography.
In: Journal of Chromatography A.
Vol. 1033
(2004)
Issue 2
.
- pp. 267-273.
ISSN 0021-9673
DOI: https://doi.org/10.1016/j.chroma.2004.01.053
Abstract in another language
Porous monoliths based on N,N-dimethylacrylamide (DMAA) or methacrylamide (MAA) were prepared inside fused silica capillaries as stationary phases for nano-chromatography. The columns were characterized in terms of flow rate and backpressure and showed, e.g. differences as a function of the salt concentration added to the polymerization mixture. When the columns were investigated for the separation of uncharged (polar hydroxylated aromatic compounds) and charged (amino acids) analytes under pressure driven conditions (pLC), differences to the previously observed behavior under voltage driven conditions (CEC) were observed. Whereas the non-charged analytes showed similar behavior in both cases—thus, corroborating the previous assumption of a mainly chromatographic separation mode driven by hydrophilic interactions in CEC—the charged amino acids did not. Assuming that the separation was governed by chromatographic phenomena in the pLC mode and by both chromatographic and electrophoretic effects in the CEC mode, the experiments allowed deconvoluting the two contributions. In particular, the charged amino acids appeared to interact with the stationary phases mainly by electrostatic interactions modified by some hydrophilic effects.
Further data
Item Type: | Article in a journal |
---|---|
Refereed: | Yes |
Keywords: | Amino acids |
Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science 500 Science > 500 Natural sciences 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering |
Date Deposited: | 25 Feb 2016 14:46 |
Last Modified: | 30 Jun 2022 09:38 |
URI: | https://eref.uni-bayreuth.de/id/eprint/31060 |