Continuous Separation of Multicomponent Protein Mixtures by Annular Displacement Chromatography

Title data

Giovannini, Roberto ; Freitag, Ruth:
Continuous Separation of Multicomponent Protein Mixtures by Annular Displacement Chromatography.
In: Biotechnology Progress. Vol. 18 (2002) Issue 6 . - pp. 1324-1331.
ISSN 1520-6033
DOI: https://doi.org/10.1021/bp010154y

Official URL:

Abstract in another language

Displacement chromatography is a predominantly nonlinear mode of chromatography, which has certain advantages over the elution mode for preparative bioseparations. Whereas continuous production (and separation) processes have their theoretical benefits in this context, protein displacement chromatography has up to now only been performed in the batch mode. In this contribution, we demonstrate that the principle of continuous annular chromatography can be adapted to displacement chromatography. Separations of up to three standard proteins (two whey proteins, soybean trypsin inhibitor) were developed and optimized using a small (4 × 250 mm) batch column. These separations were subsequently transferred directly to the continuous system (500-mL column). Separations of similar quality in terms of final product purity and recovery yield were obtained using the continuous system.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT:	No
DDC Subjects:	500 Science 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited:	25 Feb 2016 15:14
Last Modified:	25 Feb 2016 15:14
URI:	https://eref.uni-bayreuth.de/id/eprint/31067