Cell Cycle Regulation of the Murine cdc25BPromoter: Essential Role for Nuclear Factor-Y and a Proximal Repressor Element

Titelangaben

Koerner, Kathrin ; Jérôme, Valérie ; Schmidt, Thorsten ; Müller, Rolf:
Cell Cycle Regulation of the Murine cdc25BPromoter: Essential Role for Nuclear Factor-Y and a Proximal Repressor Element.
In: The Journal of Biological Chemistry. Bd. 276 (2001) Heft 13 . - S. 9662-9669.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.M008696200

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Abstract

Expression of the cdc25B gene is up-regulated late during cell cycle progression (S/G2). We have cloned the murine cdc25B promoter to identify elements involved in transcriptional regulation. A detailed structure-function analysis led to the identification of several elements that are located upstream of a canonical Inr motif at the site of transcription initiation and are involved in transcriptional activation and regulation. Activation of the promoter is largely mediated by NF-Y and Sp1/3 interacting with one and four proximal binding sites, respectively. In addition, NF-Y plays an essential role in cell cycle regulation in conjunction with a repressor element (cell cycle-regulated repressor) located ∼30 nucleotides upstream of the putative Inr element and overlapping a consensus TATA motif. The cell cycle-regulated repressor is unrelated to the previously described cell cycle-regulated repressor elements. Taken together, our observations suggest that expression of thecdc25B gene is controlled through a novel mechanism of cell cycle-regulated transcription.