Literature by the same author
plus at Google Scholar

Bibliografische Daten exportieren

On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme

Title data

Kuhn, Claus-D. ; Wilusz, Jeremy E. ; Zheng, Yuxuan ; Beal, Peter A. ; Joshua-Tor, Leemor:
On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme.
In: Cell. Vol. 160 (2015) Issue 4 . - pp. 644-658.
ISSN 0092-8674

Official URL: Volltext

Abstract in another language

Transcription in eukaryotes produces a number of long noncoding RNAs (lncRNAs). Two of these, MALAT1 and Menbeta, generate a tRNA-like small RNA in addition to the mature lncRNA. The stability of these tRNA-like small RNAs and bona fide tRNAs is monitored by the CCA-adding enzyme. Whereas CCA is added to stable tRNAs and tRNA-like transcripts, a second CCA repeat is added to certain unstable transcripts to initiate their degradation. Here, we characterize how these two scenarios are distinguished. Following the first CCA addition cycle, nucleotide binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle. Intriguingly, with the CCA-adding enzyme acting as a molecular vise, the RNAs proofread themselves through differential responses to its interrogation between stable and unstable substrates.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Archaeoglobus fulgidus/*enzymology/metabolism; Base Sequence; Catalytic Domain; Humans; Mitochondria/*enzymology/metabolism; Models, Molecular; Nucleic Acid Conformation; RNA Nucleotidyltransferases/chemistry/genetics/*metabolism; RNA Stability; RNA, Small Untranslated/metabolism; RNA, Transfer/*chemistry/*metabolism
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Profile Fields > Advanced Fields > Molecular Biosciences
Research Institutions > Research Centres > Bayreuth Center for Molecular Biosciences - BZMB
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Profile Fields
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 10 Jan 2017 10:49
Last Modified: 10 Jan 2017 10:49