at Google ScholarTitle data
Elkayam, Elad ; Kuhn, Claus-D. ; Tocilj, Ante ; Haase, Astrid D. ; Greene, Emily M. ; Hannon, Gregory J. ; Joshua-Tor, Leemor:
The structure of human argonaute-2 in complex with miR-20a.
In: Cell.
Vol. 150
(2012)
Issue 1
.
- pp. 100-110.
ISSN 1097-4172
DOI: https://doi.org/10.1016/j.cell.2012.05.017
Abstract in another language
Argonaute proteins lie at the heart of the RNA-induced silencing complex (RISC), wherein they use small RNA guides to recognize targets. Initial insight into the architecture of Argonautes came from studies of prokaryotic proteins, revealing a crescent-shaped base made up of the amino-terminal, PAZ, middle, and PIWI domains. The recently reported crystal structure of human Argonaute-2 (hAgo2), the "slicer" in RNA interference, in complex with a mixed population of RNAs derived from insect cells provides insight into the architecture of a eukaryotic Argonaute protein with defined biochemical and biological functions. Here, we report the structure of human Ago2 bound to a physiologically relevant microRNA, microRNA-20a, at 2.2 A resolution. The miRNA is anchored at both ends by the Mid and PAZ domains and makes several kinks and turns along the binding groove. Interestingly, miRNA binding confers remarkable stability on hAgo2, locking this otherwise flexible enzyme into a stable conformation.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Profile Fields > Advanced Fields > Molecular Biosciences Research Institutions > Central research institutes > Bayreuth Center for Molecular Biosciences - BZMB Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Profile Fields Profile Fields > Advanced Fields Research Institutions Research Institutions > Central research institutes |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Date Deposited: | 10 Jan 2017 10:54 |
| Last Modified: | 06 Sep 2023 11:51 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/35639 |