at Google ScholarTitle data
Hauf, Matthias ; Richter, Florian ; Schneider, Tobias ; Faidt, Thomas ; Martins, Berta M. ; Baumann, Tobias ; Durkin, Patrick ; Dobbek, Holger ; Jacobs, Karin ; Möglich, Andreas ; Budisa, Nediljko:
Photoactivatable mussel-based underwater adhesive proteins by an expanded genetic code.
In: ChemBioChem.
Vol. 18
(2017)
Issue 18
.
- pp. 1819-1823.
ISSN 1439-7633
DOI: https://doi.org/10.1002/cbic.201700327
Abstract in another language
Marine mussels exhibit potent underwater adhesion abilities under hostile conditions employing 3,4-dihydroxy-phenylalanine (Dopa)-rich mussel-adhesive proteins (MAPs). However, their recombinant production is a major biotechnological challenge. Here, we have developed a novel strategy based on genetic code expansion by engineering efficient aminoacyl-tRNA synthetases (aaRSs) for the photocaged non-canonical amino acid (ncAA) ortho-nitrobenzyl Dopa (ONB-Dopa). The engineered ONB-DopaRS enables in vivo production of MAP type 5 site-specifically equipped with multiple instances of ONB-Dopa yielding spatiotemporally controlled underwater adhesives. Upon exposure to UV light, these proteins feature elevated wet adhesion properties highlighting a novel concept for recombinant production of photocaged bioadhesives.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | aminoacyl-tRNA synthetase; atomic force microscopy based force spectroscopy; computational enzyme design; Dopa; photocaged mussel adhesive protein |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Date Deposited: | 30 Jun 2017 08:52 |
| Last Modified: | 22 Feb 2022 08:25 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/38216 |