at Google ScholarTitle data
Jérôme, Valérie ; Thoring, Lena ; Salzig, Denise ; Kubick, Stefan ; Freitag, Ruth:
Comparison of cell-based versus cell-free mammalian systems for the production of a recombinant human bone morphogenic growth factor.
In: Engineering in Life Sciences.
Vol. 17
(2017)
Issue 10
.
- pp. 1097-1107.
ISSN 1618-2863
DOI: https://doi.org/10.1002/elsc.201700005
Abstract in another language
The human bone morphogenetic protein-2 (hBMP2) is a glycoprotein, which induces de novo bone formation. Here, recombinant production in stably transfected Chinese Hamster Ovary (CHO) cells is compared to transient expression in Human Embryo Kidney (HEK) cells and cell-free synthesis in CHO cell lysates containing microsomal structures as sites of post-translational processing. In case of the stably transfected cells, growth rates and viabilities were similar to those of the parent cells, while entry into the death phase of the culture was delayed. The maximum achievable rhBMP2 concentration in these cultures was 153 pg mL−1. Up to 280 ng mL−1 could be produced in the transient expression system. In both cases the rhBMP-2 was found to interact with the producer cells, which presumably contributed to the low yields. In the cell-free system, hBMP2 yields could be increased to almost 40 μg mL−1, reached within 3 hours. The cell-free system thus approached productivities for the active (renatured) protein previously only recorded for bacterial hosts, while assuring comprehensive post-translational processing.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | Cell-free expression; CHO cell lysate; Glycosylation; hBMP2; Mammalian cell culture |
| Institutions of the University: | Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology Faculties |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 610 Medicine and health 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 20 Jul 2017 11:11 |
| Last Modified: | 09 Nov 2022 08:06 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/38736 |