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Weig, Alfons ; Franz, Juliane ; Sauer, Norbert ; Komor, Ewald:
Isolation of a family of cDNA clones from Ricinus communis L. with close homology to the hexose carriers.
In: Journal of Plant Physiology.
Bd. 143
(1994)
Heft 2
.
- S. 178-183.
ISSN 0176-1617
DOI: https://doi.org/10.1016/S0176-1617(11)81683-4
Abstract
The sugar transport of the so-called group III transport proteins, among them the two so far identified hexose carries from plants, have some strongly conserved sequences in common. Oligonucleotides coding for two conserved regions were constructed to amplify, by polymerase-chain-reaction (PCR), those mRNA species from Ricinus cotyledons that probably code for carrier proteins. Eight different sequences for putative hexose carrier proteins were identified; two of them were completed to full-length clones. Transformation into Saccharomyces cerevisiae showed that one of them definitely codes for an active glucose transport system. The other clone was, though transcribed, not expressed in yeast. The expression frequency was determined by Northern analysis and quantitative PCR. All 8 putative hexose carrier clones were present in only very small amounts in poly(A)-RNA of Ricinus tissues. The identified glucose carrier sequence (Hex3) was predominantely expressed in roots and sink leaves, whereas the other functionally so far unidentified full-length clone (Hex1) was mostly expressed in roots, hypocotyl and source leaf. Both full-length clones showed little or hardly any expression in cotyledons.
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| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Zusätzliche Informationen: | BAYCEER21863
BAYCEER122965 |
| Keywords: | sugar transport; Molecular Biology; hexose transport; Heterologous Expression; Plants; Ricinus |
| Institutionen der Universität: | Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Pflanzenphysiologie Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER Forschungseinrichtungen Forschungseinrichtungen > Forschungszentren |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 21 Mai 2014 09:17 |
| Letzte Änderung: | 12 Jul 2022 13:23 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/397 |