at Google ScholarTitle data
Graf, Christopher G. F. ; Schulz, Christian ; Schmälzlein, Marina ; Heinlein, Christian ; Mönnich, Manuel ; Perkams, Lukas ; Püttner, Markus ; Boos, Irene ; Hessefort, Markus ; Lombana Sanchez, Jose Nelson ; Weyand, Michael ; Steegborn, Clemens ; Breiden, Bernadette ; Ross, Kerstin ; Schwarzmann, Günter ; Sandhoff, Konrad ; Unverzagt, Carlo:
Synthetic Glycoforms Reveal Carbohydrate-Dependent Bioactivity of Human Saposin D.
In: Angewandte Chemie International Edition.
Vol. 56
(2017)
Issue 19
.
- pp. 5252-5257.
ISSN 1521-3773
DOI: https://doi.org/10.1002/anie.201701362
Abstract in another language
The main glycoforms of the hydrophobic lysosomal glycoprotein saposin D (SapD) were synthesized by native chemical ligation. An approach for the challenging solid-phase synthesis of the fragments was developed. Three SapD glycoforms were obtained following a general and robust refolding and purification protocol. A crystal structure of one glycoform confirmed its native structure and disulfide pattern. Functional assays revealed that the lipid-binding properties of three SapD glycoforms are highly affected by the single sugar moiety of SapD showing a dependency of the size and the type of N-glycan.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Clemens Steegborn |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
| Date Deposited: | 13 Feb 2018 08:22 |
| Last Modified: | 17 May 2022 06:36 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/42262 |