at Google ScholarTitle data
Kaiser, Patrick ; Werner, Melanie ; Jérôme, Valérie ; Freitag, Ruth:
Scale‐up of the ex vivo expansion of encapsulated primary human T lymphocytes.
In: Biotechnology and Bioengineering.
Vol. 115
(2018)
Issue 10
.
- pp. 2632-2642.
ISSN 1097-0290
DOI: https://doi.org/10.1002/bit.26786
Abstract in another language
A number of evolving medical therapies call for the controlled expansion of primary human T lymphocytes. After encapsulation in sodium cellulose sulfate–poly(diallyldimethyl) ammonium chloride polyelectrolyte capsules, T lymphocytes can be expanded without persisting activation. Here, the challenge of scaling up this process is addressed. Encapsulated T lymphocytes were cultured in spinner flasks as well as in several types of the bioreactor, including fixed and fluidized beds, a waved cell bag, and a standard stirred tank reactor (STR; 1‐L scale). Two proprietary T lymphocyte culture media as well as a standard RPMI‐based medium were used. As before, encapsulation coincided with the presence of only a low fraction of activated T lymphocytes (peripheral blood T cells) in the total population. Unexpectedly, growth rates were lower in well‐mixed reactors than those in cultivations under static conditions, that is, in T‐flasks. Switching the STR to low oxygen conditions (40% air saturation) improved the growth rates to the level of the static cultures and thus forms the potential basis for efficient scale‐up of T lymphocyte expansion. Results are presented on the ex vivo expansion of primary human T cells in T‐flasks and spinner flasks, as well as in several types of the bioreactor, including packed and fluidized beds, waved cell bags, and a standard stirred tank reactor.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Additional notes: | Online version of record before inclusion in an issue |
| Keywords: | biomimetic microenvironment; bioreactor; encapsulation; human T lymphocytes; polyelectrolyte |
| Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 570 Life sciences, biology 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 600 Technology 600 Technology, medicine, applied sciences > 610 Medicine and health |
| Date Deposited: | 25 Jul 2018 08:30 |
| Last Modified: | 27 Jan 2022 13:53 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/45236 |