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Werner, Susanne ; Hindmarsh, Patrick ; Napirei, Markus ; Vogel-Bachmayr, Karin ; Wöhrl, Birgitta M.:
Subcellular localization and integration activities of Rous Sarcoma Virus reverse transcriptase.
In: Journal of Virology.
Bd. 76
(2002)
Heft 12
.
- S. 6205-6212.
ISSN 1098-5514
DOI: https://doi.org/10.1128/JVI.76.12.6205-6212.2002
Abstract
Reverse transcriptases (RTs) αβ and β from avian Rous sarcoma virus (RSV) harbor an integrase domain which is absent in nonavian retroviral RTs. RSV integrase contains a nuclear localization signal which enables the enzyme to enter the nucleus of the cell in order to perform integration of the proviral DNA into the host genome. In the present study we analyzed the subcellular localization of RSV RT, since previous results indicated that RSV finishes synthesis of the proviral DNA in the nucleus. Our results demonstrate that the heterodimeric RSV RT αβ and the β subunit, when expressed independently, can be detected in the nucleus, whereas the separate α subunit lacking the integrase domain is prevalent in the cytoplasm. These data suggest an involvement of RSV RT in the transport of the preintegration complex into the nucleus. In addition, to analyze whether the integrase domain, located at the carboxyl terminus of β, exhibits integration activities, we investigated the nicking and joining activities of heterodimeric RSV RT αβ with an oligodeoxynucleotide-based assay system and with a donor substrate containing the supF gene flanked by the viral long terminal repeats. Our data show that RSV RT αβ is able to perform the integration reaction in vitro; however, it does so with an estimated 30-fold lower efficiency than the free RSV integrase, indicating that RSV RT is not involved in integration in vivo. Integration with RSV RT αβ could be stimulated in the presence of human immunodeficiency virus type 1 nucleocapsid protein or HMG-I(Y).
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie mit Schwerpunkt Biophysikalische Chemie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 17 Mai 2019 07:12 |
| Letzte Änderung: | 23 Aug 2022 06:47 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/48972 |