bei Google ScholarTitelangaben
Werner, Susanne ; Wöhrl, Birgitta M.:
Soluble Rous Sarcoma Virus reverse transcriptases α, αβ, and β purified from insect cells are processive DNA polymerases that lack an RNase H 3' → 5' directed processing activity.
In: The Journal of Biological Chemistry.
Bd. 274
(1999)
Heft 37
.
- S. 26329-26336.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.274.37.26329
Abstract
Reverse transcriptase (RT) isolated from Rous sarcoma virus (RSV) consists of heterodimeric RTαβ, RTα, and RTβ. The α subunit (63 kDa) contains an N-terminal polymerase and a C-terminal RNase H domain. The N terminus of β (95 kDa) corresponds to α with the integrase domain attached to the C terminus (32 kDa). We have constructed baculoviruses expressing the genes for α or β or the entire pol (99 kDa). Infection of insect cells with recombinant virus yielded highly active and soluble RSV RT enzymes that could be purified to >90% homogeneity. HPLC gel filtration showed that α is a dimeric enzyme that can be partially monomerized upon the addition of 45% Me₂SO. DNA synthesis on DNA-DNA and DNA-RNA primer-templates in the presence of competitor substrates revealed that αβ and β as well as α are processive polymerases. However, the affinity of β and αβ for primer-template substrates appears to be higher than that of α. All RSV enzymes investigated have the potential to displace RNA-RNA duplexes more efficiently than human immunodeficiency virus type 1 RT. Unlike human immunodeficiency virus type 1 RT, RSV RTs can catalyze an initial RNase H endonucleolytic cleavage of the RNA template but not a 3′ → 5′ directed processing activity.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie mit Schwerpunkt Biophysikalische Chemie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 17 Mai 2019 07:55 |
| Letzte Änderung: | 23 Aug 2022 06:49 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/48978 |