NusA Interaction with the α Subunit of E. coli RNA Polymerase Is via the UP Element Site and Releases Autoinhibition

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Schweimer, Kristian ; Prasch, Stefan J. ; Sujatha, Pagadala Santhanam ; Bubunenko, Mikhail ; Gottesman, Max E. ; Rösch, Paul:
NusA Interaction with the α Subunit of E. coli RNA Polymerase Is via the UP Element Site and Releases Autoinhibition.
In: Structure. Bd. 19 (2011) Heft 7 . - S. 945-954.
ISSN 0969-2126
DOI: https://doi.org/10.1016/j.str.2011.03.024

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Abstract

Summary Elongating Escherichia coli RNAP is modulated by NusA protein. The C-terminal domain (CTD) of the RNAP α subunit (αCTD) interacts with the acidic CTD 2 (AR2) of NusA, releasing the autoinhibitory blockade of the NusA S1-KH1-KH2 motif and allowing NusA to bind nascent nut spacer RNA. We determined the solution conformation of the AR2: αCTD complex. The αCTD residues that interface with AR2 are identical to those that recognize UP promoter elements A nusA-ΔAR2 mutation does not affect UP-dependent rrnH ...