at Google ScholarTitle data
Táncsics, András ; Farkas, Milán ; Horváth, Balázs ; Maróti, Gergely ; Bradford, Lauren M. ; Lüders, Tillmann ; Kriszt, Balázs:
Genome analysis provides insights into microaerobic toluene-degradation pathway of Zoogloea oleivorans Bucᵀ.
In: Archives of Microbiology.
Vol. 202
(2020)
.
- pp. 421-426.
ISSN 1432-072X
DOI: https://doi.org/10.1007/s00203-019-01743-8
Abstract in another language
Zoogloea oleivorans, capable of using toluene as a sole source of carbon and energy, was earlier found to be an active degrader under microaerobic conditions in aquifer samples. To uncover the genetic background of the ability of microaerobic toluene degradation in Z. oleivorans, the whole-genome sequence of the type strain BucT was revealed. Metatranscriptomic sequence reads, originated from a previous SIP study on microaerobic toluene degradation, were mapped on the genome. The genome (5.68 Mb) had a mean G + C content of 62.5%, 5005 protein coding gene sequences and 80 RNA genes. Annotation predicted that 66 genes were involved in the metabolism of aromatic compounds. Genome analysis revealed the presence of a cluster with genes coding for a multicomponent phenol-hydroxylase system and a complete catechol meta-cleavage pathway. Another cluster flanked by mobile-element protein coding genes coded a partial catechol meta-cleavage pathway including a subfamily I.2.C-type extradiol dioxygenase. Analysis of metatranscriptomic data of a microaerobic toluene-degrading enrichment, containing Z . oleivorans as an active-toluene degrader revealed that a toluene dioxygenase-like enzyme was responsible for the ring-hydroxylation, while enzymes of the partial catechol meta-cleavage pathway coding cluster were responsible for further degradation of the aromatic ring under microaerobic conditions. This further advances our understanding of aromatic hydrocarbon degradation between fully oxic and strictly anoxic conditions.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Research Institutions Research Institutions > Research Centres Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology > Chair Ecological Microbiology - Univ.-Prof. Dr. Tillmann Lüders Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science |
| Date Deposited: | 20 Jan 2020 14:12 |
| Last Modified: | 18 Jan 2022 09:47 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/53902 |