Transformation : The next level of regulation

Titelangaben

Knauer, Stefan H. ; Rösch, Paul ; Artsimovitch, Irina:
Transformation : The next level of regulation.
In: RNA Biology. Bd. 9 (2012) Heft 12 . - S. 1418-1423.
ISSN 1555-8584
DOI: https://doi.org/10.4161/rna.22724

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Abstract

The textbook view that a primary sequence determines the unique fold of a given protein has been challenged by identification of proteins with variant structures, such as prions. Our recent studies revealed that the transcription factor RfaH simultaneously changes its topology and function. RfaH is a two-domain protein whose N-terminal domain binds to transcribing RNA polymerase, stimulating its processivity. The α-helical C-terminal domain masks the RNA polymerase-binding site of the N-terminal domain, preventing unwarranted recruitment to genes lacking a specific DNA sequence. Upon binding to its DNA target, RfaH domains dissociate, and the C-terminal domain refolds into a β-barrel. This dramatic transformation allows binding to the ribosomal protein S10 and subsequent recruitment of a ribosome, coupling transcription and translation. We define RfaH as first example of "transformer proteins", in which two alternative structural states have distinct cellular functions and hypothesize that transformer proteins may be widespread in nature.