at Google ScholarTitle data
Jérôme, Valérie ; Synatschke, Christopher V. ; Freitag, Ruth:
Transient Destabilization of Biological Membranes Contributes to the Superior Performance of Star-Shaped PDMAEMA in Delivering pDNA.
In: ACS Omega.
Vol. 5
(2020)
Issue 41
.
- pp. 26640-26654.
ISSN 2470-1343
DOI: https://doi.org/10.1021/acsomega.0c03367
Abstract in another language
Nonviral DNA vectors are promising alternatives to viral ones. Their use in DNA medicine is limited by an inability to transfect, for example, nondividing or suspension cells. In recent years, star-shaped synthetic polycationic vectors, so called “Nanostars”, have shown some promise in this regard, at least when compared to the “gold standard” in nonviral vectors, namely, linear poly(ethyleneimine) (l-PEI). It has been hypothesized that an ability to transiently destabilize cellular membranes is partially responsible for the phenomenon. This hypothesis is investigated here, taking human leukemia suspension cells (Jurkat cells) as an example. Contrary to l-PEI, the Nanostars promote the cellular uptake of small, normally membrane-impermeant molecules (trypan blue and propidium iodide) as well as that of fluorescent polystyrene beads (average diameter 100 nm). Since Nanostars, but not l-PEI, are apparently able to deliver DNA to nuclei of nondividing cells, nuclear uptake is, in addition, investigated with isolated cell nuclei. Our results provide evidence that Nanostars are more efficient than l-PEI in increasing the nuclear membrane association/permeability, allowing accumulation of their cargo on/in the nucleus.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Process Biotechnology |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 570 Life sciences, biology 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 600 Technology 600 Technology, medicine, applied sciences > 610 Medicine and health |
| Date Deposited: | 15 Oct 2020 11:24 |
| Last Modified: | 09 Nov 2022 08:09 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/58391 |