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Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF

Titelangaben

Gorbovytska, Vladyslava ; Kim, Seung-Kyoon ; Kuybu, Filiz ; Götze, Michael ; Um, Dahun ; Kang, Keunsoo ; Pittroff, Andreas ; Schneider, Lisa-Marie ; Leitner, Alexander ; Kim, Tae-Kyung ; Kuhn, Claus-D.:
Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF.
bioRxiv , 2021
DOI: https://doi.org/10.1101/2021.04.25.441328

Volltext

Link zum Volltext (externe URL): Volltext

Angaben zu Projekten

Projekttitel:
Offizieller Projekttitel
Projekt-ID
Are eRNAs displacing NELF from paused RNA polymerase and thereby activate transcription ?
Ohne Angabe

Projektfinanzierung: Deutsche Forschungsgemeinschaft
KU3514/1-1

Abstract

Enhancer RNAs (eRNAs) are long non-coding RNAs that originate from enhancers. Although eRNA transcription is a canonical feature of activated enhancers, the molecular features required for eRNA function and the mechanism of how eRNAs impinge on target gene transcription have not been established. Thus, using eRNA-dependent RNA polymerase II (Pol II) pause release as a model, we examined the requirement of sequence, structure and length of eRNAs for their ability to stimulate Pol II pause release by detaching NELF from paused Pol II. We found eRNA not to exert their function through common structural or sequence motifs. Instead, efficient NELF release requires a single eRNA molecule that must contain unpaired guanosines to make multiple, allosteric contacts with several NELF subunits. By revealing the molecular determinants for eRNA function, our study mechanistically links eRNAs to Pol II pause release and provides new insight into the regulation of metazoan transcription.

Weitere Angaben

Publikationsform: Preprint, Postprint
Institutionen der Universität: Profilfelder > Advanced Fields > Molekulare Biowissenschaften
Graduierteneinrichtungen > University of Bayreuth Graduate School
Graduierteneinrichtungen > Elitenetzwerk Bayern > Structural Basis of Gene Regulation by Non-coding RNA
Profilfelder
Profilfelder > Advanced Fields
Graduierteneinrichtungen
Graduierteneinrichtungen > Elitenetzwerk Bayern
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
Eingestellt am: 04 Jun 2021 09:58
Letzte Änderung: 23 Aug 2023 13:28
URI: https://eref.uni-bayreuth.de/id/eprint/65661