Title data
Gorbovytska, Vladyslava ; Kim, Seung-Kyoon ; Kuybu, Filiz ; Götze, Michael ; Um, Dahun ; Kang, Keunsoo ; Pittroff, Andreas ; Schneider, Lisa-Marie ; Leitner, Alexander ; Kim, Tae-Kyung ; Kuhn, Claus-D.:
Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF.
bioRxiv
,
2021
DOI: https://doi.org/10.1101/2021.04.25.441328
Project information
Project title: |
Project's official title Project's id Are eRNAs displacing NELF from paused RNA polymerase and thereby activate transcription ? No information |
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Project financing: |
Deutsche Forschungsgemeinschaft KU3514/1-1 |
Abstract in another language
Enhancer RNAs (eRNAs) are long non-coding RNAs that originate from enhancers. Although eRNA transcription is a canonical feature of activated enhancers, the molecular features required for eRNA function and the mechanism of how eRNAs impinge on target gene transcription have not been established. Thus, using eRNA-dependent RNA polymerase II (Pol II) pause release as a model, we examined the requirement of sequence, structure and length of eRNAs for their ability to stimulate Pol II pause release by detaching NELF from paused Pol II. We found eRNA not to exert their function through common structural or sequence motifs. Instead, efficient NELF release requires a single eRNA molecule that must contain unpaired guanosines to make multiple, allosteric contacts with several NELF subunits. By revealing the molecular determinants for eRNA function, our study mechanistically links eRNAs to Pol II pause release and provides new insight into the regulation of metazoan transcription.
Further data
Item Type: | Preprint, postprint |
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Institutions of the University: | Profile Fields > Advanced Fields > Molecular Biosciences Graduate Schools > University of Bayreuth Graduate School Graduate Schools > Elite Network Bavaria > Structural Basis of Gene Regulation by Non-coding RNA Profile Fields Profile Fields > Advanced Fields Graduate Schools Graduate Schools > Elite Network Bavaria |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science 500 Science > 570 Life sciences, biology |
Date Deposited: | 04 Jun 2021 09:58 |
Last Modified: | 23 Aug 2023 13:28 |
URI: | https://eref.uni-bayreuth.de/id/eprint/65661 |