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Using Blinking Optical Tweezers to Study Cell Rheology During Initial Cell-Particle Contact

Titelangaben

Berghoff, Konrad ; Gross, Wolfgang ; Eisentraut, Manuel ; Kress, Holger:
Using Blinking Optical Tweezers to Study Cell Rheology During Initial Cell-Particle Contact.
In: Biophysical Journal. Bd. 120 (2021) Heft 16 . - S. 3527-3537.
ISSN 1542-0086
DOI: https://doi.org/10.1016/j.bpj.2021.04.034

Angaben zu Projekten

Projekttitel:
Offizieller Projekttitel
Projekt-ID
SFB 1357 Mikroplastik
SFB1357
KR 3524/4-1
KR 3524/4-1

Projektfinanzierung: Deutsche Forschungsgemeinschaft
Studienstiftung des deutschen Volkes
Elite Network of Bavaria

Abstract

Phagocytosis is an important part of innate immunity and describes the engulfment of bacteria and other extracellular objects on the micrometer-scale. The protrusion of the cell membrane around the bacteria during this process is driven by a reorganization of the actin cortex. The process has been studied on the molecular level to great extent during the past decades. However, a deep, fundamental understanding of the mechanics of the process is still lacking, in particular due to a lack of techniques which give access to binding dynamics below the optical resolution limit and cellular viscoelasticity at the same time. In this work, we propose a technique to characterize the mechanical properties of cells in a highly localized manner and apply it to investigate the early stages of phagocytosis. The technique can simultaneously resolve the contact region between a cell and an external object (in our application a phagocytic target) even below the optical resolution limit. We used immunoglobulin-G (IgG)-coated microparticles with a size of 2 μm as a model system and attached the particles to the macrophages with holographic optical tweezers. By switching the trap on and off, we were able to measure the rheological properties of the cells in a time-resolved manner during the first few minutes after attachment. The measured viscoelastic cellular response is consistent with power law rheology. The contact radius between particle and cell increased on a timescale of about 30 s and converged after a few minutes. While the binding dynamics are not affected by cytochalasin D, we observed an increase of the cellular compliance and a significant fluidization of the cortex after addition of cytochalasin D treatment. Furthermore, we report upper boundaries for the length- and timescale, at which cortical actin has been hypothesized to depolymerize during early phagocytosis.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten
Fakultäten > Fakultät für Mathematik, Physik und Informatik
Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut
Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut > Professur Experimentalphysik VI - Biologische Physik
Profilfelder > Advanced Fields > Polymer- und Kolloidforschung
Profilfelder > Advanced Fields > Molekulare Biowissenschaften
Profilfelder > Advanced Fields > Nichtlineare Dynamik
Forschungseinrichtungen
Forschungseinrichtungen > Sonderforschungsbereiche, Forschergruppen
Forschungseinrichtungen > Sonderforschungsbereiche, Forschergruppen > SFB 1357 - MIKROPLASTIK
Profilfelder
Profilfelder > Advanced Fields
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften
500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
Eingestellt am: 06 Jul 2021 13:50
Letzte Änderung: 25 Apr 2022 13:21
URI: https://eref.uni-bayreuth.de/id/eprint/66462