Title data
Dietler, Julia ; Schubert, Roman ; Krafft, Tobias G. A. ; Meiler, Simone ; Kainrath, Stephanie ; Richter, Florian ; Schweimer, Kristian ; Weyand, Michael ; Janovjak, Harald ; Möglich, Andreas:
A Light-Oxygen-Voltage Receptor Integrates Light and Temperature.
In: Journal of Molecular Biology.
Vol. 433
(2021)
Issue 15
.
- No. 167107.
ISSN 0022-2836
DOI: https://doi.org/10.1016/j.jmb.2021.167107
Project information
Project financing: |
Alexander von Humboldt-Stiftung Deutsche Forschungsgemeinschaft |
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Abstract in another language
Sensory photoreceptors enable organisms to adjust their physiology, behavior, and development in response to light, generally with spatiotemporal acuity and reversibility. These traits underlie the use of photoreceptors as genetically encoded actuators to alter by light the state and properties of heterologous organisms. Subsumed as optogenetics, pertinent approaches enable regulating diverse cellular processes, not least gene expression. Here, we controlled the widely used Tet repressor by coupling to light-oxygen-voltage (LOV) modules that either homodimerize or dissociate under blue light. Repression could thus be elevated or relieved, and consequently protein expression was modulated by light. Strikingly, the homodimeric RsLOV module from Rhodobacter sphaeroides not only dissociated under light but intrinsically reacted to temperature. The limited light responses of wild-type RsLOV at 37 °C were enhanced in two variants that exhibited closely similar photochemistry and structure. One variant improved the weak homodimerization affinity of 40 µM by two-fold and thus also bestowed light sensitivity on a receptor tyrosine kinase. Certain photoreceptors, exemplified by RsLOV, can evidently moonlight as temperature sensors which immediately bears on their application in optogenetics and biotechnology. Properly accounted for, the temperature sensitivity can be leveraged for the construction of signal-responsive cellular circuits.
Further data
Item Type: | Article in a journal |
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Refereed: | Yes |
Keywords: | crystal structure; optogenetics; sensory photoreceptor; signal transduction; Tet repressor |
Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich Research Institutions > Research Centres > Nordbayerisches Zentrum für NMR-Spektroskopie - NMR-Zentrum Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry Research Institutions Research Institutions > Research Centres |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science > 570 Life sciences, biology |
Date Deposited: | 16 Jul 2021 09:23 |
Last Modified: | 11 Jul 2022 13:29 |
URI: | https://eref.uni-bayreuth.de/id/eprint/66559 |