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Spider Silk Capsules as Protective Reaction Containers for Enzymes

Title data

Blüm, Claudia ; Nichtl, Alfons ; Scheibel, Thomas:
Spider Silk Capsules as Protective Reaction Containers for Enzymes.
In: Advanced Functional Materials. (2014) Issue 24 . - pp. 763-768.
ISSN 1616-3028

Abstract in another language

Spider silk fi bres are well known for their high tensile strength in combination with high elasticity. Based on the possibility of recombinant production of spider silk proteins, technical applications of spider silk materials are nowadays feasible. The engineered recombinant spider silk protein eADF4(C16) is based on the sequence of ADF4 ( Araneus diadematus fi broin), one out of at least three proteins of the dragline silk of the European garden spider A. diadematus. The protein eADF4(C16) can be processed into different morphologies. Here, capsules of eADF4(C16) are assembled at an oil/water interface. These microcapsules are mechanically stable and can be used as a transport
system for higher molecular weight compounds such as enzymes or chemical catalysts. Further, they can be regarded as a small enclosed reaction chamber with a semi-permeable membrane. Reactions can be initiated by diffusion of the reactants through the silk membrane. The eADF4(C16) capsules protect the enzyme β -galactosidase, used as model, against proteolysis. Functional α-complementation of β -galactosidase visualizes the controllable activation of an enzyme within such spider silk capsule, highlighting the broad applicability there of as reaction containers, e.g., for enzymes.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: capsules; spider silks; β-galactosidase; protease protection; enzymes
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Emerging Fields > Food and Health Sciences
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Emerging Fields
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 11 Feb 2015 13:32
Last Modified: 01 Feb 2021 10:00