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De novo designed peptides for cellular delivery and subcellular localisation

Title data

Rhys, Guto G. ; Cross, Jessica A. ; Dawson, William M. ; Thompson, Harry F. ; Shanmugaratnam, Sooruban ; Savery, Nigel J. ; Dodding, Mark P. ; Höcker, Birte ; Woolfson, Derek N.:
De novo designed peptides for cellular delivery and subcellular localisation.
In: Nature Chemical Biology. Vol. 18 (2022) Issue 9 . - pp. 999-1004.
ISSN 1552-4469
DOI: https://doi.org/10.1038/s41589-022-01076-6

Project information

Project financing: Andere

Abstract in another language

Increasingly, it is possible to design peptide and protein assemblies de novo from first principles or computationally. This approach provides new routes to functional synthetic polypeptides, including designs to target and bind proteins of interest. Much of this work has been developed in vitro. Therefore, a challenge is to deliver de novo polypeptides efficiently to sites of action within cells. Here we describe the design, characterisation, intracellular delivery, and subcellular localisation of a de novo synthetic peptide system. This system comprises a dual-function basic peptide, programmed both for cell penetration and target binding, and a complementary acidic peptide that can be fused to proteins of interest and introduced into cells using synthetic DNA. The designs are characterised in vitro using biophysical methods and X-ray crystallography. The utility of the system for delivery into mammalian cells and subcellular targeting is demonstrated by marking organelles and actively engaging functional protein complexes.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Birte Höcker
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 09 Dec 2022 06:24
Last Modified: 09 Dec 2022 06:24
URI: https://eref.uni-bayreuth.de/id/eprint/72980