Title data
Rhys, Guto G. ; Cross, Jessica A. ; Dawson, William M. ; Thompson, Harry F. ; Shanmugaratnam, Sooruban ; Savery, Nigel J. ; Dodding, Mark P. ; Höcker, Birte ; Woolfson, Derek N.:
De novo designed peptides for cellular delivery and subcellular localisation.
In: Nature Chemical Biology.
Vol. 18
(2022)
Issue 9
.
- pp. 999-1004.
ISSN 1552-4469
DOI: https://doi.org/10.1038/s41589-022-01076-6
Project information
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Abstract in another language
Increasingly, it is possible to design peptide and protein assemblies de novo from first principles or computationally. This approach provides new routes to functional synthetic polypeptides, including designs to target and bind proteins of interest. Much of this work has been developed in vitro. Therefore, a challenge is to deliver de novo polypeptides efficiently to sites of action within cells. Here we describe the design, characterisation, intracellular delivery, and subcellular localisation of a de novo synthetic peptide system. This system comprises a dual-function basic peptide, programmed both for cell penetration and target binding, and a complementary acidic peptide that can be fused to proteins of interest and introduced into cells using synthetic DNA. The designs are characterised in vitro using biophysical methods and X-ray crystallography. The utility of the system for delivery into mammalian cells and subcellular targeting is demonstrated by marking organelles and actively engaging functional protein complexes.
Further data
Item Type: | Article in a journal |
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Refereed: | Yes |
Institutions of the University: | Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Birte Höcker Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science 500 Science > 500 Natural sciences 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
Date Deposited: | 09 Dec 2022 06:24 |
Last Modified: | 09 Dec 2022 06:24 |
URI: | https://eref.uni-bayreuth.de/id/eprint/72980 |