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Investigation of the halophilic PET hydrolase PET6 from Vibrio gazogenes

Title data

Weigert, Sebastian ; Perez‐Garcia, Pablo ; Gisdon, Florian J. ; Gagsteiger, Andreas ; Schweinshaut, Kristine ; Ullmann, G. Matthias ; Chow, Jennifer ; Streit, Wolfgang R. ; Höcker, Birte:
Investigation of the halophilic PET hydrolase PET6 from Vibrio gazogenes.
In: Protein Science. Vol. 31 (2022) Issue 12 . - e4500.
ISSN 1469-896X
DOI: https://doi.org/10.1002/pro.4500

Official URL: Volltext

Project information

Project financing: Bundesministerium für Bildung und Forschung
Deutsche Forschungsgemeinschaft

Abstract in another language

The handling of plastic waste and the associated ubiquitous occurrence of microplastic poses one of the biggest challenges of our time. Recent investigations of plastic degrading enzymes have opened new prospects for biological microplastic decomposition as well as recycling applications. For polyethylene terephthalate, in particular, several natural and engineered enzymes are known to have such promising properties. From a previous study that identified new PETase candidates by homology search, we chose the candidate PET6 from the globally distributed, halophilic organism Vibrio gazogenes for further investigation. By mapping the occurrence of Vibrios containing PET6 homologs we demonstrated their ubiquitous prevalence in the pangenome of several Vibrio strains. The biochemical characterization of PET6 showed that PET6 has a comparatively lower activity than other enzymes but also revealed a superior turnover at very high salt concentrations. The crystal structure of PET6 provides structural insights into this adaptation to saline environments. By grafting only a few beneficial mutations from other PET degrading enzymes onto PET6, we increased the activity up to three-fold, demonstrating the evolutionary potential of the enzyme. MD simulations of the variant helped rationalize the mutational effects of those mutants and elucidate the interaction of the enzyme with a PET substrate. With tremendous amounts of plastic waste in the Ocean and the prevalence of Vibrio gazogenes in marine biofilms and estuarine marshes, our findings suggest that Vibrio and the PET6 enzyme are worthy subjects to study the PET degradation in marine environments.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: MD simulation; PET degradation; PETase; hydrolases; metagenome; polyethylene terephthalate; substrate interaction
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Birte Höcker
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Research Institutions > Collaborative Research Centers, Research Unit > SFB 1357 - MIKROPLASTIK
Research Institutions
Research Institutions > Collaborative Research Centers, Research Unit
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 09 Dec 2022 06:46
Last Modified: 13 Dec 2023 11:10
URI: https://eref.uni-bayreuth.de/id/eprint/72983