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Surface immobilization of viruses and nanoparticles elucidates early events in clathrin-mediated endocytosis

Title data

Fratini, Marta ; Wiegand, Tina ; Funaya, Charlotta ; Jiang, Zhongxiang ; Shah, Pranav N. M. ; Spatz, Joachim P. ; Cavalcanti-Adam, Elisabetta Ada ; Boulant, Steeve:
Surface immobilization of viruses and nanoparticles elucidates early events in clathrin-mediated endocytosis.
In: ACS Infectious Diseases. Vol. 4 (2018) Issue 11 . - pp. 1585-1600.
ISSN 2373-8227

Abstract in another language

Clathrin-mediated endocytosis (CME) is an important entry pathway for viruses. Here, we applied click chemistry to covalently immobilize reovirus on surfaces to study CME during early host–pathogen interactions. To uncouple chemical and physical properties of viruses and determine their impact on CME initiation, we used the same strategy to covalently immobilize nanoparticles of different sizes. Using fluorescence live microscopy and electron microscopy, we confirmed that clathrin recruitment depends on particle size and discovered that the maturation into clathrin-coated vesicles (CCVs) is independent from cargo internalization. Surprisingly, we found that the final size of CCVs appears to be imprinted on the clathrin coat at early stages of cargo–cell interactions. Our approach has allowed us to unravel novel aspects of early interactions between viruses and the clathrin machinery that influence late stages of CME and CCVs formation. This method can be easily and broadly applied to the field of nanotechnology, endocytosis, and virology.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: viruses; nanoparticles; endocytosis; clathrin-mediated endocytosis; membrane curvature; click chemistry
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam
Result of work at the UBT: No
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 07 Jun 2023 13:11
Last Modified: 07 Jun 2023 13:11