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Jahnke, Kevin ; Maurer, Stefan J. ; Weber, Cornelia ; Hernandez Bücher, Jochen Estebano ; Schoenit, Andreas ; D'Este, Elisa ; Cavalcanti-Adam, Elisabetta Ada ; Göpfrich, Kerstin:
Actomyosin-Assisted Pulling of Lipid Nanotubes from Lipid Vesicles and Cells.
In: Nano Letters.
Bd. 22
(2022)
Heft 3
.
- S. 1145-1150.
ISSN 1530-6992
DOI: https://doi.org/10.1021/acs.nanolett.1c04254
Abstract
Molecular motors are pivotal for intracellular transport as well as cell motility and have great potential to be put to use outside cells. Here, we exploit engineered motor proteins in combination with self-assembly of actin filaments to actively pull lipid nanotubes from giant unilamellar vesicles (GUVs). In particular, actin filaments are bound to the outer GUV membrane and the GUVs are seeded on a heavy meromyosin-coated substrate. Upon addition of ATP, hollow lipid nanotubes with a length of tens of micrometer are pulled from single GUVs due to the motor activity. We employ the same mechanism to pull lipid nanotubes from different types of cells. We find that the length and number of nanotubes critically depends on the cell type, whereby suspension cells form bigger networks than adherent cells. This suggests that molecular machines can be used to exert forces on living cells to probe membrane-to-cortex attachment.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | Lipid nanotubes; lipid tether pulling; motility assay; giant unilamellar vesicle; membrane-to-cortex attachment; actin; heavy mero-myosin |
| Institutionen der Universität: | Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Zelluläre Biomechanik > Lehrstuhl Zelluläre Biomechanik - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam Fakultäten Fakultäten > Fakultät für Ingenieurwissenschaften Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Zelluläre Biomechanik |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 07 Jun 2023 06:26 |
| Letzte Änderung: | 07 Jun 2023 06:26 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/81233 |