at Google ScholarTitle data
Jahnke, Kevin ; Maurer, Stefan J. ; Weber, Cornelia ; Hernandez Bücher, Jochen Estebano ; Schoenit, Andreas ; D'Este, Elisa ; Cavalcanti-Adam, Elisabetta Ada ; Göpfrich, Kerstin:
Actomyosin-Assisted Pulling of Lipid Nanotubes from Lipid Vesicles and Cells.
In: Nano Letters.
Vol. 22
(2022)
Issue 3
.
- pp. 1145-1150.
ISSN 1530-6992
DOI: https://doi.org/10.1021/acs.nanolett.1c04254
Abstract in another language
Molecular motors are pivotal for intracellular transport as well as cell motility and have great potential to be put to use outside cells. Here, we exploit engineered motor proteins in combination with self-assembly of actin filaments to actively pull lipid nanotubes from giant unilamellar vesicles (GUVs). In particular, actin filaments are bound to the outer GUV membrane and the GUVs are seeded on a heavy meromyosin-coated substrate. Upon addition of ATP, hollow lipid nanotubes with a length of tens of micrometer are pulled from single GUVs due to the motor activity. We employ the same mechanism to pull lipid nanotubes from different types of cells. We find that the length and number of nanotubes critically depends on the cell type, whereby suspension cells form bigger networks than adherent cells. This suggests that molecular machines can be used to exert forces on living cells to probe membrane-to-cortex attachment.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | Lipid nanotubes; lipid tether pulling; motility assay; giant unilamellar vesicle; membrane-to-cortex attachment; actin; heavy mero-myosin |
| Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics |
| Result of work at the UBT: | No |
| DDC Subjects: | 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 07 Jun 2023 06:26 |
| Last Modified: | 07 Jun 2023 06:26 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/81233 |