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Oliver-Cervelló, Lluís ; Martín-Gómez, Helena ; Reyes, Leslie ; Noureddine, Fatima ; Cavalcanti-Adam, Elisabetta Ada ; Ginebra, Maria-Pau ; Mas-Moruno, Carlos:
An engineered biomimetic peptide regulates cell behavior by synergistic integrin and growth factor signaling.
In: Advanced Healthcare Materials.
Bd. 10
(2021)
Heft 7
.
- 2001757.
ISSN 2192-2659
DOI: https://doi.org/10.1002/adhm.202001757
Abstract
Recreating the healing microenvironment is essential to regulate cell–material interactions and ensure the integration of biomaterials. To repair bone, such bioactivity can be achieved by mimicking its extracellular matrix (ECM) and by stimulating integrin and growth factor (GF) signaling. However, current approaches relying on the use of GFs, such as bone morphogenetic protein 2 (BMP-2), entail clinical risks. Here, a biomimetic peptide integrating the RGD cell adhesive sequence and the osteogenic DWIVA motif derived from the wrist epitope of BMP-2 is presented. The approach offers the advantage of having a spatial control over the single binding of integrins and BMP receptors. Such multifunctional platform is designed to incorporate 3,4-dihydroxyphenylalanine to bind metallic oxides with high affinity in a one step process. Functionalization of glass substrates with the engineered peptide is characterized by physicochemical methods, proving a successful surface modification. The biomimetic interfaces significantly improve the adhesion of C2C12 cells, inhibit myotube formation, and activate the BMP-dependent signaling via p38. These effects are not observed on surfaces displaying only one bioactive motif, a mixture of both motifs or soluble DWIVA. These data prove the biological potential of recreating the ECM and engaging in integrin and GF crosstalk via molecular-based mimics.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Zelluläre Biomechanik > Lehrstuhl Zelluläre Biomechanik - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam Fakultäten Fakultäten > Fakultät für Ingenieurwissenschaften Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Zelluläre Biomechanik |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 07 Jun 2023 06:33 |
| Letzte Änderung: | 24 Okt 2023 14:09 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/81240 |