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Hohmann, Urszula ; Cardinal von Widdern, Julian ; Ghadban, Chalid ; Lo Giudice, Maria Cristina ; Lemahieu, Grégoire ; Cavalcanti-Adam, Elisabetta Ada ; Dehghani, Faramarz ; Hohmann, Tim:
Jamming Transitions in Astrocytes and Glioblastoma Are Induced by Cell Density and Tension.
In: Cells.
Bd. 12
(2023)
Heft 1
.
- 29.
ISSN 2073-4409
DOI: https://doi.org/10.3390/cells12010029
Abstract
Collective behavior of cells emerges from coordination of cell–cell-interactions and is important to wound healing, embryonic and tumor development. Depending on cell density and cell–cell interactions, a transition from a migratory, fluid-like unjammed state to a more static and solid-like jammed state or vice versa can occur. Here, we analyze collective migration dynamics of astrocytes and glioblastoma cells using live cell imaging. Furthermore, atomic force microscopy, traction force microscopy and spheroid generation assays were used to study cell adhesion, traction and mechanics. Perturbations of traction and adhesion were induced via ROCK or myosin II inhibition. Whereas astrocytes resided within a non-migratory, jammed state, glioblastoma were migratory and unjammed. Furthermore, we demonstrated that a switch from an unjammed to a jammed state was induced upon alteration of the equilibrium between cell–cell-adhesion and tension from adhesion to tension dominated, via inhibition of ROCK or myosin II. Such behavior has implications for understanding the infiltration of the brain by glioblastoma cells and may help to identify new strategies to develop anti-migratory drugs and strategies for glioblastoma-treatment.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | glioblastoma; migration; collective migration; jamming; unjamming; adhesion; tension; astrocytes |
| Institutionen der Universität: | Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Zelluläre Biomechanik > Lehrstuhl Zelluläre Biomechanik - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 07 Jun 2023 06:35 |
| Letzte Änderung: | 07 Jun 2023 06:35 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/81243 |