at Google ScholarTitle data
Hohmann, Urszula ; Cardinal von Widdern, Julian ; Ghadban, Chalid ; Lo Giudice, Maria Cristina ; Lemahieu, Grégoire ; Cavalcanti-Adam, Elisabetta Ada ; Dehghani, Faramarz ; Hohmann, Tim:
Jamming Transitions in Astrocytes and Glioblastoma Are Induced by Cell Density and Tension.
In: Cells.
Vol. 12
(2023)
Issue 1
.
- 29.
ISSN 2073-4409
DOI: https://doi.org/10.3390/cells12010029
Abstract in another language
Collective behavior of cells emerges from coordination of cell–cell-interactions and is important to wound healing, embryonic and tumor development. Depending on cell density and cell–cell interactions, a transition from a migratory, fluid-like unjammed state to a more static and solid-like jammed state or vice versa can occur. Here, we analyze collective migration dynamics of astrocytes and glioblastoma cells using live cell imaging. Furthermore, atomic force microscopy, traction force microscopy and spheroid generation assays were used to study cell adhesion, traction and mechanics. Perturbations of traction and adhesion were induced via ROCK or myosin II inhibition. Whereas astrocytes resided within a non-migratory, jammed state, glioblastoma were migratory and unjammed. Furthermore, we demonstrated that a switch from an unjammed to a jammed state was induced upon alteration of the equilibrium between cell–cell-adhesion and tension from adhesion to tension dominated, via inhibition of ROCK or myosin II. Such behavior has implications for understanding the infiltration of the brain by glioblastoma cells and may help to identify new strategies to develop anti-migratory drugs and strategies for glioblastoma-treatment.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | glioblastoma; migration; collective migration; jamming; unjamming; adhesion; tension; astrocytes |
| Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam |
| Result of work at the UBT: | No |
| DDC Subjects: | 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 07 Jun 2023 06:35 |
| Last Modified: | 07 Jun 2023 06:35 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/81243 |