Title data
Buheitel, Johannes ; Stemmann, Olaf:
Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate.
In: The EMBO Journal.
Vol. 32
(2013)
Issue 5
.
- pp. 666-676.
ISSN 1460-2075
DOI: https://doi.org/10.1038/emboj.2013.7
Abstract in another language
Faithful transmission of chromosomes during eukaryotic cell division requires sister chromatids to be paired from their generation in S phase until their separation in M phase. Cohesion is mediated by the cohesin complex, whose Smc1, Smc3 and Scc1 subunits form a tripartite ring that entraps both DNA double strands. Whereas centromeric cohesin is removed in late metaphase by Scc1 cleavage, metazoan cohesin at chromosome arms is displaced already in prophase by proteolysis-independent signalling. Which of the three gates is triggered by the prophase pathway to open has remained enigmatic. Here, we show that displacement of human cohesin from early mitotic chromosomes requires dissociation of Smc3 from Scc1 but no opening of the other two gates. In contrast, loading of human cohesin onto chromatin in telophase occurs through the Smc1-Smc3 hinge. We propose that the use of differently regulated gates for loading and release facilitates unidirectionality of DNA's entry into and exit from the cohesin ring
Further data
Item Type: | Article in a journal |
---|---|
Refereed: | Yes |
Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics > Chair Genetics - Univ.-Prof. Dr. Olaf Stemmann Profile Fields > Advanced Fields > Molecular Biosciences Research Institutions > Central research institutes > Bayreuth Center for Molecular Biosciences - BZMB Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics Profile Fields Profile Fields > Advanced Fields Research Institutions Research Institutions > Central research institutes |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science > 570 Life sciences, biology |
Date Deposited: | 26 Mar 2015 07:59 |
Last Modified: | 15 Jun 2023 11:54 |
URI: | https://eref.uni-bayreuth.de/id/eprint/8385 |