at Google ScholarTitle data
Orth, Michael ; Mayer, Bernd ; Rehm, Kinga ; Rothweiler, Ulli ; Heidmann, Doris ; Holak, Tad A. ; Stemmann, Olaf:
Shugoshin is a Mad1/Cdc20-like interactor of Mad2.
In: The EMBO Journal.
Vol. 30
(2011)
.
- pp. 2868-2880.
ISSN 1460-2075
DOI: https://doi.org/10.1038/emboj.2011.187
Abstract in another language
Mammalian centromeric cohesin is protected from phosphorylation-dependent displacement in mitotic prophase by shugoshin-1 (Sgo1), while shugoshin-2 (Sgo2) protects cohesin from separase-dependent cleavage in meiosis I. In higher eukaryotes, progression and faithful execution of both mitosis and meiosis are controlled by the spindle assembly checkpoint, which delays anaphase onset until chromosomes have achieved proper attachment to microtubules. According to the so-called template model, Mad1-Mad2 complexes at unattached kinetochores instruct conformational change of soluble Mad2, thus catalysing Mad2 binding to its target Cdc20. Here, we show that human Sgo2, but not Sgo1, specifically interacts with Mad2 in a manner that strongly resembles the interactions of Mad2 with Mad1 or Cdc20. Sgo2 contains a Mad1/Cdc20-like Mad2-interaction motif and competes with Mad1 and Cdc20 for binding to Mad2. NMR and biochemical analyses show that shugoshin binding induces similar conformational changes in Mad2 as do Mad1 or Cdc20. Mad2 binding regulates fine-tuning of Sgo2's sub-centromeric localization. Mad2 binding is conserved in the only known Xenopus laevis shugoshin homologue and, compatible with a putative meiotic function, the interaction occurs in oocytes
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics > Chair Genetics - Univ.-Prof. Dr. Olaf Stemmann Profile Fields > Advanced Fields > Molecular Biosciences Research Institutions > Central research institutes > Bayreuth Center for Molecular Biosciences - BZMB Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics Profile Fields Profile Fields > Advanced Fields Research Institutions Research Institutions > Central research institutes |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Date Deposited: | 26 Mar 2015 09:56 |
| Last Modified: | 15 Jun 2023 11:53 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/8418 |