Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1

Title data

Hormanseder, Eva ; Tischer, Thomas ; Heubes, Simone ; Stemmann, Olaf ; Mayer, Thomas U.:
Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1.
In: EMBO Reports. Vol. 12 (12 May 2011) Issue 5 . - pp. 436-443.
ISSN 1469-3178
DOI: https://doi.org/10.1038/embor.2011.32

Official URL:

Abstract in another language

Mature Xenopus oocytes are arrested in meiosis by the activity of XErp1/Emi2, an inhibitor of the ubiquitin-ligase anaphase-promoting complex/cyclosome (APC/C). On fertilization, XErp1 is degraded, resulting in APC/C activation and the consequent degradation of cell-cycle regulators and exit from meiosis. In this study, we show that a modest increase in the activity of the ubiquitin-conjugating enzyme UbcX overrides the meiotic arrest in an APC/C-dependent reaction. Intriguingly, XErp1 remains stable in these conditions. We found that UbcX causes the ubiquitylation of XErp1, followed by its dissociation from the APC/C. Our data support the idea that ubiquitylation regulates the APC/C-inhibitory activity of XErp1.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics > Chair Genetics - Univ.-Prof. Dr. Olaf Stemmann Profile Fields > Advanced Fields > Molecular Biosciences Research Institutions > Research Centres > Bayreuth Center for Molecular Biosciences - BZMB Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics Profile Fields Profile Fields > Advanced Fields Research Institutions Research Institutions > Research Centres
Result of work at the UBT:	Yes
DDC Subjects:	500 Science > 570 Life sciences, biology
Date Deposited:	26 Mar 2015 09:59
Last Modified:	26 Mar 2015 09:59
URI:	https://eref.uni-bayreuth.de/id/eprint/8432