Essential Cdk1-inhibitory role for separase during meiosis I in vertebrate oocytes

Title data

Gorr, Ingo H. ; Reis, Alexandra ; Boos, Dominik ; Wühr, Martin ; Madgwick, Suzanne ; Jones, Keith T. ; Stemmann, Olaf:
Essential Cdk1-inhibitory role for separase during meiosis I in vertebrate oocytes.
In: Nature Cell Biology. Vol. 8 (8 September 2006) . - pp. 1035-1037.
ISSN 1476-4679
DOI: https://doi.org/10.1038/ncb1467

Official URL:

Abstract in another language

Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics > Chair Genetics - Univ.-Prof. Dr. Olaf Stemmann Profile Fields > Advanced Fields > Molecular Biosciences Research Institutions > Research Centres > Bayreuth Center for Molecular Biosciences - BZMB Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics Profile Fields Profile Fields > Advanced Fields Research Institutions Research Institutions > Research Centres
Result of work at the UBT:	No
DDC Subjects:	500 Science > 570 Life sciences, biology
Date Deposited:	26 Mar 2015 11:35
Last Modified:	26 Mar 2015 11:35
URI:	https://eref.uni-bayreuth.de/id/eprint/8546