at Google ScholarTitle data
Huschek, Gerd ; Rawel, Harshadrai M. ; Schweikert, Torsten ; Henkel-Oberländer, Janin ; Tchewonpi Sagu, Sorel:
Characterization and optimization of microwave-assisted extraction of B-phycoerythrin from Porphyridium purpureum using response surface methodology and Doehlert design.
In: Bioresource Technology Reports.
Vol. 19
(September 2022)
.
- 101212.
ISSN 2589-014X
DOI: https://doi.org/10.1016/j.biteb.2022.101212
Abstract in another language
Microalgae are one of the most promising food source of the future. Nowadays, extracts of high-value active substances of biomass are business aims for the development of food additives in personalized nutrition, in cosmetics and pharmaceuticals. A new-patented vertical farming cultivation technology was used for production of Porphyridium purpureum. In this work, microwave assisted extraction was used to extract B-phycoerythrin from Porphyridium purpureum biomass. Response surface methodology was implemented for optimization. Numerical optimization established the best point of the experimental domain (biomass/solvent of 16.8 mg/mL, time of 172 s, and temperature of 30 °C) with a desirability value of 0.82. Corresponding experimental responses values of 7.2 mg, 8.5 % and 13,961 PA/μg biomass were obtained for extracted proteins, extraction yield and extracted B-phycoerythrin, respectively. Final freeze-dried product indicated protein content of 55 % using Kjeldahl while targeted mass spectrometry analysis revealed that B-phycoerythrin represented 93 % of the total protein.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Life Sciences: Food, Nutrition and Health Faculties > Faculty of Life Sciences: Food, Nutrition and Health > Lehrstuhl Biochemie der Ernährung Faculties > Faculty of Life Sciences: Food, Nutrition and Health > Lehrstuhl Biochemie der Ernährung > Lehrstuhl Biochemie der Ernährung - Univ.-Prof. Dr. Janin Henkel-Oberländer |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
| Date Deposited: | 18 Sep 2023 08:49 |
| Last Modified: | 18 Sep 2023 08:49 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/86875 |