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Kitana, Waseem ; Levario Diaz, Victoria ; Cavalcanti-Adam, Elisabetta Ada ; Ionov, Leonid:
Biofabrication of Composite Bioink-Nanofiber Constructs : Effect of Rheological Properties of Bioinks on 3D (Bio)Printing and Cells Interaction with Aligned Touch Spun Nanofibers.
In: Advanced Healthcare Materials.
Bd. 13
(2024)
Heft 6
.
- 2303343.
ISSN 2192-2659
DOI: https://doi.org/10.1002/adhm.202303343
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Angaben zu Projekten
| Projekttitel: |
Offizieller Projekttitel Projekt-ID Fabrikation von Mikrofasern mit komplexem Interieur durch formverändernde Polymere 396913955 TRR 225: Von den Grundlagen der Biofabrikation zu funktionalen Gewebemodellen 326998133 |
|---|---|
| Projektfinanzierung: |
Deutsche Forschungsgemeinschaft |
Abstract
This paper reports on a novel approach for the fabrication of composite multilayered bioink-nanofibers construct. This work achieves this by using a hands-free 3D (bio)printing integrated touch-spinning approach. Additionally, this work investigates the interaction of fibroblasts in different bioinks with the highly aligned touch-spun nanofibers. This work conducts a comprehensive characterization of the rheological properties of the inks, starting with low-strain oscillatory rheology to analyze the viscoelastic behavior, when the material structure remains intact. Moreover, this work performs amplitude sweeps to investigate the stability of the inks under large deformations, rotational rheology to examine the shear thinning profile, and a three-step creep experiment to study time-dependent rheological behavior. The obtained rheological results are correlated to visual observation of the flow behavior of inks. These behaviors span from an ink with zero-shear viscosity, very weak shear thinning, and no thixotropic behavior to inks exhibiting flow stress, pronounced shear thinning, and thixotropy. It is demonstrated that inks have an essential effect on cell behavior. While all bioinks allow a preferred directionality of the fibroblasts along the fiber direction, cells tend to form aggregates in bioinks with higher viscosity, and a considerable number of agglomerates are observed in the presence of laponite-RD.