bei Google ScholarTitelangaben
D'Ermo, Giulia ; Audebert, Stéphane ; Camoin, Luc ; Planer-Friedrich, Britta ; Casiot‐Marouani, Corinne ; Delpoux, Sophie ; Lebrun, Régine ; Guiral, Marianne ; Schoepp‐Cothenet, Barbara:
Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila.
In: Environmental Microbiology.
Bd. 26
(2024)
Heft 6
.
- e16655.
ISSN 1462-2920
DOI: https://doi.org/10.1111/1462-2920.16655
Abstract
The metabolic process of purple sulphur bacteria’s anoxygenic photosynthesis has been primarily studied in Allochromatium vinosum, a member of the Chromatiaceae family. However, the metabolic processes of purple sul-
phur bacteria from the Ectothiorhodospiraceae and Halorhodospiraceae families remain unexplored. We have analysed the proteome of Halorhodospira halophila, a member of the Halorhodospiraceae family, which was cultivated with various sulphur compounds. This analysis allowed us to
reconstruct the first comprehensive sulphur-oxidative photosynthetic network for this family. Some members of the Ectothiorhodospiraceae family have been shown to use arsenite as a photosynthetic electron donor. Therefore, we analysed the proteome response of Halorhodospira halophila when grown under arsenite and sulphide conditions. Our analyses using ion chromatography-inductively coupled plasma mass spectrometry showed that thioarsenates are chemically formed under these conditions. However, they are more extensively generated and converted in the presence of bac-
teria, suggesting a biological process. Our quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced under these growth conditions. Additionally, two electron car-
riers, cytochrome c551 /c5 and HiPIP III, are also overproduced. Electron paramagnetic resonance spectroscopy suggested that these transporters participate in the reduction of the photosynthetic Reaction Centre. These
results support the idea of a chemically and biologically formed thioarsenate being oxidized by the Sox system, with cytochrome c551 /c5 and HiPIP III directing electrons towards the Reaction Centre.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Geowissenschaften > Professur Umweltgeochemie Forschungseinrichtungen > Zentrale wissenschaftliche Einrichtungen > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 550 Geowissenschaften, Geologie |
| Eingestellt am: | 24 Jun 2024 09:20 |
| Letzte Änderung: | 24 Jun 2024 09:20 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/89819 |