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Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila

Title data

D'Ermo, Giulia ; Audebert, Stéphane ; Camoin, Luc ; Planer-Friedrich, Britta ; Casiot‐Marouani, Corinne ; Delpoux, Sophie ; Lebrun, Régine ; Guiral, Marianne ; Schoepp‐Cothenet, Barbara:
Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila.
In: Environmental Microbiology. Vol. 26 (2024) Issue 6 . - e16655.
ISSN 1462-2920

Abstract in another language

The metabolic process of purple sulphur bacteria’s anoxygenic photosynthesis has been primarily studied in Allochromatium vinosum, a member of the Chromatiaceae family. However, the metabolic processes of purple sul-
phur bacteria from the Ectothiorhodospiraceae and Halorhodospiraceae families remain unexplored. We have analysed the proteome of Halorhodospira halophila, a member of the Halorhodospiraceae family, which was cultivated with various sulphur compounds. This analysis allowed us to
reconstruct the first comprehensive sulphur-oxidative photosynthetic network for this family. Some members of the Ectothiorhodospiraceae family have been shown to use arsenite as a photosynthetic electron donor. Therefore, we analysed the proteome response of Halorhodospira halophila when grown under arsenite and sulphide conditions. Our analyses using ion chromatography-inductively coupled plasma mass spectrometry showed that thioarsenates are chemically formed under these conditions. However, they are more extensively generated and converted in the presence of bac-
teria, suggesting a biological process. Our quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced under these growth conditions. Additionally, two electron car-
riers, cytochrome c551 /c5 and HiPIP III, are also overproduced. Electron paramagnetic resonance spectroscopy suggested that these transporters participate in the reduction of the photosynthetic Reaction Centre. These
results support the idea of a chemically and biologically formed thioarsenate being oxidized by the Sox system, with cytochrome c551 /c5 and HiPIP III directing electrons towards the Reaction Centre.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Professor Environmental Geochemistry Group
Research Institutions > Central research institutes > Bayreuth Center of Ecology and Environmental Research- BayCEER
Result of work at the UBT: No
DDC Subjects: 500 Science > 550 Earth sciences, geology
Date Deposited: 24 Jun 2024 09:20
Last Modified: 24 Jun 2024 09:20