Literature by the same author
plus at Google Scholar

Bibliografische Daten exportieren
 

Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila

Title data

D'Ermo, Giulia ; Audebert, Stéphane ; Camoin, Luc ; Planer-Friedrich, Britta ; Casiot‐Marouani, Corinne ; Delpoux, Sophie ; Lebrun, Régine ; Guiral, Marianne ; Schoepp‐Cothenet, Barbara:
Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila.
In: Environmental Microbiology. Vol. 26 (2024) Issue 6 . - e16655.
ISSN 1462-2920
DOI: https://doi.org/10.1111/1462-2920.16655

Abstract in another language

The metabolic process of purple sulphur bacteria’s anoxygenic photosynthesis has been primarily studied in Allochromatium vinosum, a member of the Chromatiaceae family. However, the metabolic processes of purple sul-
phur bacteria from the Ectothiorhodospiraceae and Halorhodospiraceae families remain unexplored. We have analysed the proteome of Halorhodospira halophila, a member of the Halorhodospiraceae family, which was cultivated with various sulphur compounds. This analysis allowed us to
reconstruct the first comprehensive sulphur-oxidative photosynthetic network for this family. Some members of the Ectothiorhodospiraceae family have been shown to use arsenite as a photosynthetic electron donor. Therefore, we analysed the proteome response of Halorhodospira halophila when grown under arsenite and sulphide conditions. Our analyses using ion chromatography-inductively coupled plasma mass spectrometry showed that thioarsenates are chemically formed under these conditions. However, they are more extensively generated and converted in the presence of bac-
teria, suggesting a biological process. Our quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced under these growth conditions. Additionally, two electron car-
riers, cytochrome c551 /c5 and HiPIP III, are also overproduced. Electron paramagnetic resonance spectroscopy suggested that these transporters participate in the reduction of the photosynthetic Reaction Centre. These
results support the idea of a chemically and biologically formed thioarsenate being oxidized by the Sox system, with cytochrome c551 /c5 and HiPIP III directing electrons towards the Reaction Centre.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Professor Environmental Geochemistry Group
Research Institutions > Central research institutes > Bayreuth Center of Ecology and Environmental Research- BayCEER
Result of work at the UBT: No
DDC Subjects: 500 Science > 550 Earth sciences, geology
Date Deposited: 24 Jun 2024 09:20
Last Modified: 24 Jun 2024 09:20
URI: https://eref.uni-bayreuth.de/id/eprint/89819