Titelangaben
Chamas, Alexandre ; Svensson, Carl-Magnus ; Maneira, Carla ; Sporniak, Marta ; Figge, Marc Thilo ; Lackner, Gerald:
Engineering adhesion of the probiotic strain Escherichia coli Nissle to the fungal pathogen Candida albicans.
In: ACS Synthetic Biology.
Bd. 13
(2024)
Heft 12
.
- S. 4027-4039.
ISSN 2161-5063
DOI: https://doi.org/10.1021/acssynbio.4c00466
Abstract
Engineering live biotherapeutic products against fungal pathogens such as Candida albicans has been suggested as a means to tackle the increasing threat of fungal infections and the development of resistance to classical antifungal treatments. One important challenge in the design of live therapeutics is to control their localization inside the human body. The specific binding capability to target organisms or tissues would greatly increase their effectiveness by increasing the local concentration of effector molecules at the site of infection. In this study, we utilized surface display of carbohydrate binding domains to enable the probiotic E. coli Nissle 1917 to adhere specifically to the pathogenic yeast Candida albicans. Binding was quantified using a newly developed method based on the automated analysis of microscopic images. In addition to a rationally selected chitin binding domain, a synthetic peptide of identical length but distinct sequence also conferred binding. Efficient binding was specific to fungal hyphae, the invasive form of C. albicans, while the yeast form, as well as abiotic cellulose and PET particles, was only weakly recognized.
Weitere Angaben
Publikationsform: | Artikel in einer Zeitschrift |
---|---|
Begutachteter Beitrag: | Ja |
Keywords: | fungal pathogens; Candida albicans; live therapeutics; probiotic E. coli Nissle |
Institutionen der Universität: | Fakultäten > Fakultät für Lebenswissenschaften: Lebensmittel, Ernährung und Gesundheit Fakultäten > Fakultät für Lebenswissenschaften: Lebensmittel, Ernährung und Gesundheit > Lehrstuhl Biochemie der Mikroorganismen > Lehrstuhl Biochemie der Mikroorganismen - Univ.-Prof. Dr. Gerald Lackner |
Titel an der UBT entstanden: | Nein |
Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
Eingestellt am: | 15 Jan 2025 08:12 |
Letzte Änderung: | 15 Jan 2025 08:34 |
URI: | https://eref.uni-bayreuth.de/id/eprint/91584 |