at Google ScholarTitle data
Chamas, Alexandre ; Svensson, Carl-Magnus ; Maneira, Carla ; Sporniak, Marta ; Figge, Marc Thilo ; Lackner, Gerald:
Engineering adhesion of the probiotic strain Escherichia coli Nissle to the fungal pathogen Candida albicans.
In: ACS Synthetic Biology.
Vol. 13
(2024)
Issue 12
.
- pp. 4027-4039.
ISSN 2161-5063
DOI: https://doi.org/10.1021/acssynbio.4c00466
Abstract in another language
Engineering live biotherapeutic products against fungal pathogens such as Candida albicans has been suggested as a means to tackle the increasing threat of fungal infections and the development of resistance to classical antifungal treatments. One important challenge in the design of live therapeutics is to control their localization inside the human body. The specific binding capability to target organisms or tissues would greatly increase their effectiveness by increasing the local concentration of effector molecules at the site of infection. In this study, we utilized surface display of carbohydrate binding domains to enable the probiotic E. coli Nissle 1917 to adhere specifically to the pathogenic yeast Candida albicans. Binding was quantified using a newly developed method based on the automated analysis of microscopic images. In addition to a rationally selected chitin binding domain, a synthetic peptide of identical length but distinct sequence also conferred binding. Efficient binding was specific to fungal hyphae, the invasive form of C. albicans, while the yeast form, as well as abiotic cellulose and PET particles, was only weakly recognized.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | fungal pathogens; Candida albicans; live therapeutics; probiotic E. coli Nissle |
| Institutions of the University: | Faculties > Faculty of Life Sciences: Food, Nutrition and Health Faculties > Faculty of Life Sciences: Food, Nutrition and Health > Chair Biochemistry of Microorganisms > Chair Biochemistry of Microorganisms - Univ.-Prof. Dr. Gerald Lackner |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
| Date Deposited: | 15 Jan 2025 08:12 |
| Last Modified: | 15 Jan 2025 08:34 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/91584 |