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Varsadiya, Milan ; Dehghani, Fatemeh ; Yang, Shiyue ; Blagodatskaya, Evgenia ; Maskow, Thomas ; Meier, Dimitri ; Lüders, Tillmann:
Carbon and energy utilization in microbial cell extracts from soil.
In: European Journal of Soil Biology.
Bd. 124
(2025)
.
- 103713.
ISSN 1164-5563
DOI: https://doi.org/10.1016/j.ejsobi.2025.103713
Abstract
Microbial carbon use efficiency (CUE), the ratio of carbon retained in biomass vs. total C uptake, is central to our understanding of organic C turnover in soil. A precise quantification of CUE in soils can be challenging, given the considerable analytical uncertainties of organic and inorganic C backgrounds. At the same time, CUE measured for model pure cultures will be distinct from a diverse microbiota in soil. As a proxy between laboratory cultures and complex soil microbiomes, we tested soil-free microbial cell extracts (SFCE) to unravel patterns of C utilization in soil-derived microbiomes of reduced complexity. For this, we have revisited and optimized established protocols to extract microbial cells from agricultural soil via Nycodenz density centrifugation. The total extracted cells were quantified, accounting for up to ∼3.5 × 107 cells g−1 soil and representing ∼12.5 % of the original soil microbiome. The diversity of microbes in SFCE, while consistently reduced compared to soil, still retained a surprisingly high proportion of the original soil microbiome, with ASVs recovered from 21 phyla. We then inferred CUE from calorespirometric measurements (metabolic heat flow and CO2 production) to compare values between SFCE and intact soil. Both were amended with substrates (glucose, glutamine, and glycerol) of different C and N content, and C oxidation state (NOSC). SFCE showed CUE values principally comparable to that of the intact soil, but with substrate-specific distinctions. Amplicon sequencing and qPCR-based quantification showed typical soil taxa like Pseudomonas, Pseudarthrobacter, and Bacteroidota to respond to substrate addition in soil and SFCE. Our results support the use of SFCE as a valuable and complementary approach toward elucidating microbial CUE and growth patterns for complex soil microbiota.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Ökologische Mikrobiologie > Lehrstuhl Ökologische Mikrobiologie - Univ.-Prof. Dr. Tillmann Lüders |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 11 Mär 2025 06:34 |
| Letzte Änderung: | 11 Mär 2025 06:34 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/92751 |